Promoter Engineering Reveals the Importance of Heptameric Direct Repeats for DNA Binding by Streptomyces Antibiotic Regulatory Protein–Large ATP-Binding Regulator of the LuxR Family (SARP-LAL) Regulators in Streptomyces natalensis - INSA Toulouse - Institut National des Sciences Appliquées de Toulouse Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2018

Promoter Engineering Reveals the Importance of Heptameric Direct Repeats for DNA Binding by Streptomyces Antibiotic Regulatory Protein–Large ATP-Binding Regulator of the LuxR Family (SARP-LAL) Regulators in Streptomyces natalensis

Eva Barreales
  • Function : Author
Cláudia M Vicente
Antonio de Pedro
  • Function : Author
Javier Santos-Aberturas
  • Function : Author
Jesús Aparicio

Abstract

The biosynthesis of small-size polyene macrolides is ultimately con-trolled by acouple of transcriptional regulators that act in a hierarchical way. AStreptomycesantibiotic regulatory protein–large ATP-binding regulator of the LuxRfamily (SARP-LAL) regulator binds the promoter of a PAS-LuxR regulator-encodinggene and activates its transcription, and in turn, the gene product of the latter acti-vates transcription from various promoters of the polyene gene cluster directly. Theprimary operator of PimR, the archetype of SARP-LAL regulators, contains three hep-tameric directrepeats separated by four-nucleotide spacers, but the regulator canalso bind a secondary operator with only two direct repeats separated by a3-nucleotide spacer, both located in the promoter region of its unique target gene,pimM. A similar arrangement of operators has been identified for PimR counterpartsencoded by gene clusters for different antifungal secondary metabolites, includingnot only polyene macrolides but peptidyl nucleosides, phoslactomycins, or cyclohex-imide. Here, we used promoter engineering and quantitative transcriptional analysesto determine the contributions of the different heptameric repeats to transcriptionalactivation and final polyene production. Optimized promoters have thus been devel-oped. Deletion studies and electrophoretic mobility assays were used for the defini-tion of DNA-binding boxes formed by 22-nucleotide sequences comprising twoconserved heptameric direct repeats separated by four-nucleotide less conservedspacers. The cooperative binding of PimRSARPappears to be the mechanism involvedin the binding of regulator monomers to operators, and at least two protein mono-mers are required for efficient binding.

Dates and versions

hal-02562727 , version 1 (04-05-2020)

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Eva Barreales, Cláudia M Vicente, Antonio de Pedro, Javier Santos-Aberturas, Jesús Aparicio. Promoter Engineering Reveals the Importance of Heptameric Direct Repeats for DNA Binding by Streptomyces Antibiotic Regulatory Protein–Large ATP-Binding Regulator of the LuxR Family (SARP-LAL) Regulators in Streptomyces natalensis. Applied and Environmental Microbiology, 2018, 84 (10), ⟨10.1128/AEM.00246-18⟩. ⟨hal-02562727⟩
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