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Journal Articles Enzyme and Microbial Technology Year : 2000

An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

L. Brambilla
  • Function : Author
P. Duboc
  • Function : Author
M.L.F. Giuseppin
  • Function : Author
J.J. Heijnen
  • Function : Author
M. Hoare
  • Function : Author
H.C. Lange
  • Function : Author
E.A. Madden
  • Function : Author
P. Niederberger
  • Function : Author
J. Nielsen
  • Function : Author
D. Porro
  • Function : Author
M. Reuss
  • Function : Author
N van Riel
  • Function : Author
M. Rizzi
  • Function : Author
H.Y. Steensma
  • Function : Author
C.T. Verrips
  • Function : Author
J. Vindeløv
  • Function : Author

Abstract

To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
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Dates and versions

hal-02559361 , version 1 (30-04-2020)
hal-02559361 , version 2 (05-05-2020)

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J.P. Van Dijken, J. Bauer, L. Brambilla, P. Duboc, J.M. Francois, et al.. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme and Microbial Technology, 2000, 26 (9-10), pp.706-714. ⟨10.1016/s0141-0229(00)00162-9⟩. ⟨hal-02559361v2⟩
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