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Journal Articles Applied and Environmental Microbiology Year : 2019

Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli

Abstract

In this work, we shed light on the metabolism of dihydroxyacetone(DHA), a versatile, ubiquitous, and important intermediate for various chemicals inindustry, by analyzing its metabolism at the system level in Escherichia coli. Using constraint-based modeling, we show that the growth of E. coli on DHA is suboptimal and identify the potential causes. Nuclear magnetic resonance analysis shows thatDHA is degraded nonenzymatically into substrates known to be unfavorable to highgrowth rates. Transcriptomic analysis reveals that DHA promotes genes involved in biofilm formation, which may reduce the bacterial growth rate. Functional analysis of the genes involved in DHA metabolism proves that under the aerobic conditions used in this study, DHA is mainly assimilated via the dihydroxyacetone kinase path-way. In addition, these results show that the alternative routes of DHA assimilation(i.e., the glycerol and fructose-6-phosphate aldolase pathways) are not fully activated under our conditions because of anaerobically mediated hierarchical control. These pathways are therefore certainly unable to sustain fluxes as high as the ones pre-dictedin silicofor optimal aerobic growth on DHA. Overexpressing some of thegenes in these pathways releases these constraints and restores the predicted opti-mal growth on DHA.
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Dates and versions

hal-02194204 , version 1 (13-12-2023)

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Camille Peiro, Pierre Millard, Alessandro de Simone, Edern Cahoreau, Lindsay Peyriga, et al.. Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli. Applied and Environmental Microbiology, 2019, 85 (15), pp.1-17. ⟨10.1128/AEM.00768-19⟩. ⟨hal-02194204⟩
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