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Journal Articles Biotechnology Letters Year : 2000

An intracellular metabolite quantification technique applicable to polysaccharide-producing bacteria

Abstract

An experimental procedure for the quantification of intracellular concentrations of metabolites in exoploysacharide-producing bacteria has been developed. This simple technique is based on the simultaneous quenching and extraction of metabolites using cold ethanol. Extracellular polysaccharide is precipitated with the cell matter generating clean samples which can be further concentrated using evaporation techniques or solid state extraction columns. Intracellular pools, coherent with the operation of the Entner–Doudoroff pathway were observed in Xanthomonas campestris.
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Dates and versions

hal-02183612 , version 1 (15-07-2019)

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  • HAL Id : hal-02183612 , version 1

Cite

Fabien Létisse, N.D. Lindley. An intracellular metabolite quantification technique applicable to polysaccharide-producing bacteria. Biotechnology Letters, 2000, 22 (21), pp.1673-1677. ⟨hal-02183612⟩
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