Carbon flux analysis in a pantothenate overproducing Corynebacterium glutamicum strain. - INSA Toulouse - Institut National des Sciences Appliquées de Toulouse Access content directly
Journal Articles Molecular Biology Reports Year : 2003

Carbon flux analysis in a pantothenate overproducing Corynebacterium glutamicum strain.

Abstract

Carbon flux analysis during a pseudo-stationary phase of metabolite accumulation in a genetically engineered strain of Corynebacterium glutamicum, containing plasmids leading to over-expression of the ilvBNCD and panBC operons, has identified the basic metabolic constraints governing the potential of this bacterium to produce pantothenate. Carbon flux converging on pyruvate (75% of glucose uptake) is controlled by anabolic precursor requirements and NADPH demand provoking high carbon loss as CO2 via the pentose pathway. Virtually all the flux of pyruvate is directed into the branched pathway leading to both valine and pantothenate production, but flux towards valine is tenfold higher than that transformed to pantothenate, indicating that significant improvements will only be obtained if carbon flux at the ketoisovalerate branchpoint can be modulated.

Dates and versions

hal-02183327 , version 1 (15-07-2019)

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Cite

Christophe Chassagnole, Fabien Letisse, Audrey Diano, Nic Lindley. Carbon flux analysis in a pantothenate overproducing Corynebacterium glutamicum strain.. Molecular Biology Reports, 2003, 29 (1-2), pp.129-134. ⟨10.1023/A:1020353124066⟩. ⟨hal-02183327⟩
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