Application of 2D-TOCSY NMR to the measurement of specific 13C-enrichments in complex mixtures of 13C-labeled metabolites - INSA Toulouse - Institut National des Sciences Appliquées de Toulouse Access content directly
Journal Articles Metabolic Engineering Year : 2007

Application of 2D-TOCSY NMR to the measurement of specific 13C-enrichments in complex mixtures of 13C-labeled metabolites

Abstract

A 2D-NMR method based on zero-quantum filtered (ZQF-) TOtal Correlation SpectroscopY (TOCSY) was applied to measure 13C-enrichments in complex mixtures of 13C-labeled metabolites generated in carbon-labeling experiments. Using ZQF-TOCSY, more than 30 13C-enrichments could be potentially measured from the analysis of a biomass hydrolyzate prepared from Escherichia coli cells grown on a mixture of 20% [U-13C]-glucose and 80% [1-13C]-glucose, without need for separation of metabolites. The method is applicable to biomass hydrolyzates, cell extracts, and other complex biological samples. It is also applicable to any combination of labeled substrates and provides a basis for examining non-steady-state conditions.

Dates and versions

hal-02183153 , version 1 (15-07-2019)

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Cite

S. Massou, S. Nicolas, Jean-Charles Portais, Fabien Létisse. Application of 2D-TOCSY NMR to the measurement of specific 13C-enrichments in complex mixtures of 13C-labeled metabolites. Metabolic Engineering, 2007, 9 (3), pp.252-257. ⟨10.1016/j.ymben.2007.03.001⟩. ⟨hal-02183153⟩
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