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Article Dans Une Revue Applied Microbiology and Biotechnology Année : 2002

Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process

Résumé

Several bottlenecks in the alcoholic fermentation process must be overcome to reach a very high and competitive performance of bioethanol production by the yeast Saccharomyces cerevisiae. In this paper, a nutritional strategy is described that allowed S. cerevisiae to produce a final ethanol titre of 19% (v/v) ethanol in 45 h in a fed-batch culture at 30°C. This performance was achieved by implementing exponential feeding of vitamins throughout the fermentation process. In comparison to an initial addition of a vitamin cocktail, an increase in the amount of vitamins and an exponential vitamin feeding strategy improved the final ethanol titre from 126 g l–1 to 135 g l–1 and 147 g l–1, respectively. A maximum instantaneous productivity of 9.5 g l–1 h–1 was reached in the best fermentation. These performances resulted from improvements in growth, the specific ethanol production rate, and the concentration of viable cells in response to the nutritional strategy.

Dates et versions

hal-02169999 , version 1 (01-07-2019)

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Sandrine Alfenore, Carole Molina-Jouve, Stéphane Guillouet, Jean-Louis Uribelarrea, Gérard Goma, et al.. Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process. Applied Microbiology and Biotechnology, 2002, 60 (1-2), pp.67-72. ⟨10.1007/s00253-002-1092-7⟩. ⟨hal-02169999⟩
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