Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process - INSA Toulouse - Institut National des Sciences Appliquées de Toulouse Access content directly
Journal Articles Applied Microbiology and Biotechnology Year : 2004

Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process

Abstract

In order to identify an optimal aeration strategy for intensifying bio-fuel ethanol production in fermentation processes where growth and production have to be managed simultaneously, we quantified the effect of aeration conditions—oxygen limited vs non limited culture (micro-aerobic vs aerobic culture)—on the dynamic behaviour of Saccharomyces cerevisiae cultivated in very high ethanol performance fed-batch cultures. Fermentation parameters and kinetics were established within a range of ethanol concentrations (up to 147 g l−1), which very few studies have addressed. Higher ethanol titres (147 vs 131 g l−1 in 45 h) and average productivity (3.3 vs 2.6 g l−1 h−1) were obtained in cultures without oxygen limitation. Compared to micro-aerobic culture, full aeration led to a 23% increase in the viable cell mass as a result of the concomitant increase in growth rate and yield, with lower ethanol inhibition. The second beneficial effect of aeration was better management of by-product production, with production of glycerol, the main by-product, being strongly reduced from 12 to 4 g l−1. We demonstrate that aeration strategy is as much a determining factor as vitamin feeding (Alfenore et al. 2002) in very high ethanol performance (147 g l−1 in 45 h) in order to achieve a highly competitive dynamic process.

Dates and versions

hal-02169957 , version 1 (01-07-2019)

Identifiers

Cite

Sandrine Alfenore, Xavier Cameleyre, Laurent Benbadis, Carine Bideaux, Jean-Louis Uribelarrea, et al.. Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process. Applied Microbiology and Biotechnology, 2004, 63 (5), pp.537-542. ⟨10.1007/s00253-003-1393-5⟩. ⟨hal-02169957⟩
56 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More